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Partridges exposed to diquat during development showed paler red traits than controls (Fig. 2; diquat treatment: F1,61 = 5.63, P = 0.020; red reference chip F1,57.7 = 145.7, P<0.001).
All gene expression data were normalized to a standard reference chip using the dCHIP software.
Curves for binding to a blank reference chip were subtracted using the software package Biaevaluation (GE Healthcare).
Feathers were first scanned in colour at 1500 dpi with a yellow reference chip (hue = 40, chroma = 78).
To determine enrichment, the 2-ΔΔCp method was used by comparing enrichment values for positive primer pairs to a negative primer pair between experimental and reference ChIP experiments.
To account for differences in the scans, hue and chroma measures were standardized against the standard reference chip, and these residual colour values were used in all analyses.
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The Java application includes tools to calculate signal ratios against one or more reference chips with a variety of options available to fine-tune the analysis.
The current functionality of the application includes methods to extract signals into a single tab-delimited file for subsets of features and GC-matched background signal, normalization procedures, routines to generate signal ratios against one or more reference chips with options to fine-tune analyses and standard or user-defined annotation files for merging analyzed signal.
Moreover, a precise analog reference has been provided to the microcontroller by means of an LM4132 precision voltage references chip in order to provide stable and accurate analog measures [40].
The influence of the anisotropy of bone to the orthogonal cutting was studied in reference to chip formation and thus, considered in the model to explain the chip morphologies in different cutting directions relative to the bone fibres (i.e. osteon).
Although yesterday's speech made only a single reference to chip and PIN, the technology is arguably the most crucial element in reforming the country's data security policies and practices.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com