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The device consists of a reference chamber (R) and a microwave irradiation chamber (I), of 0.5 m3 volume each one, equipped with access doors.
The calibration factors were determined using solutions whose activity concentration is traceable to the international reference system using the IRA reference chamber.
In course of the measurements the temperature was stepwise increased by 0.5 °C/min, while the heat flux in the sample chamber and reference chamber is measured.
First, crushed sample method is the simplest method where only two chambers of known volumes are needed and gas is allowed to expand into the crushed sample chamber from a reference chamber (Cui et al. 2009; Zamirian et al. 2014).
However, the reference chamber was filled with the same bacterial suspension as the test chamber but without adding antibiotic.
NIR profiles for medium with antibiotic (sterile medium only in reference chamber) and NIR profiles for untreated cells in both chambers served as controls for these experiments.
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The partial pressure difference between the experimental and reference chambers represented the PenH parameter, and the data presented as the percent increase above baseline PenH measurements.
In each cycle, each measurement chamber was active for at least 110 seconds (first and reference chambers were active 10 sec longer each) during which O2 values were sampled every 1 sec.
The results were compared with those of a reference cylindrical chamber.
The experimentally determined RAKR of HDR 192Ir sources, using this reference ionization chamber by applying the analytically estimated NK, was found to be in agreement with the vendor quoted RAKR within 1.43%.
Instead it was the thousands of antisemitic tweets unleashed on Twitter by Spanish fans, referencing gas chambers and the Holocaust.
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