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A reference blood sample was created by adding 22.9 ± 15.7 MBq (at the time of administration, range: 10.1 to 49.5 MBq of 11C-TMSX in approximately 60 μL to this blood sample and incubated for 10 min at 37°C).
The syringe pump for extraction of the arterial reference blood sample was started with a speed of 0.5 mL/min and continued over 2 min. The microspheres were injected 0.5 min after starting the reference blood sampling with a rate of 1 mL/min.
The median week for the reference blood sample was week 17 (range 11 162).
For CV calculation, one reference blood sample was measured five times on each plate across all 10 plates.
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The coefficient of correlation between continuously withdrawn microdialysed blood and venously taken reference blood samples was r = 0.9834 (0.9834–0.9834).
Reference blood samples were dried on filter paper (Carl Roth GmbH + Co. Kg, Germany) and analysed using the technique mentioned above.
Reference blood samples were drawn from the pulmonary artery through the Swan Ganz into a heparinized syringe at a rate of 5 ml/min (for 3 min) with an automated syringe pump (Harvard PHD 22/2000 Advance Syringe Pump, Harvard Apparatus), starting 1 min before MS injection.
Reference blood samples were kindly provided by two male individuals of the Department of Biological Sciences, University of Naples Federico II, where this work was carried out.
Two hundred-ten reference blood samples were collected.
Reference blood samples were taken from either a peripheral vein, a central venous catheter or an arterial line.
Reference blood samples were taken from the radial artery and internal jugular bulb vein, at baseline and after a series of increasingly hypoxic states induced by altering the inspired oxygen concentration while maintaining normocapnic arterial carbon dioxide pressure (Pa co2).
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