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The reference-dependent alignment accuracy is calculated as the percentage of correctly aligned positions judged by reference alignments, which are generated by structural alignment programs.
The reference-dependent accuracy is defined as the percentage of correctly aligned positions judged by the reference alignments.
Among the obtained alignments, those with TM-scores >0.6 for Fr-TM-align and those with Z-scores >2 for DaliLite were used as reference alignments, respectively.
The scoring function was parameterized by reproducing reference alignments taken from four different proteins.
Consequently, it is not trivial to know the quality of MSAs when reference alignments are not provided.
The context of our study is an ontology network consisting of several taxonomies networked by partial reference alignments.
We have tested our method on the state-of-the-art secondary structure prediction methods PHD, PROFsec, SSPro2 and JNET using the HOMSTRAD database of reference alignments.
This raised the question whether COACH analysis should routinely be performed with all three reference alignments.
This allowed us to assemble meaningful reference alignments composed exclusively of established rather than predicted repeats.
The alignments obtained from each of the 8 programs were compared to the corresponding reference alignments.
Thus 24% of HEAT repeats were detected with only one, or two, of the reference alignments.
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