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For the reference alignment, however, one has to estimate ω using some inference method.
Assuming that one has a good reference alignment, the greengenes aligner appears to have the most potential for quickly generating a high quality sequence alignment.
The reference alignment had 72 aligned pairs.
Third, unaligned sequences could then be aligned to the reference alignment using a variety of parameters.
Sequences were aligned using the Silva reference alignment as a template and potentially chimeric sequences were eliminated using the UChime algorithm34.
The reads were aligned to the Greengenes Core reference alignment using PyNAST 56.
The agreement to a reference alignment was computed as the percentage of residues aligned, with a tolerance shift of s positions, to the reference alignment (I s ) relative to the length of the reference alignment (Lref): I s / Lref.
To align a group of sequences to a reference alignment, we used the "--add" option of MAFFT [ 94].
For each reference alignment, blocks are defined that correspond to the reliably aligned regions, using the RASCAL [60] program.
We use TM-align (Zhang and Skolnick, 2005b) to build a reference alignment for a protein pair in SALIGN.
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The particles were windowed into individual 96×96 pixels images, rotationally and translationally aligned, and subjected to 10 cycles of multi-reference alignment.
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