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The mass spectrometric data collected were used to search the most recent non-redundant protein database using ProtTech's proprietary software suite; and the relative abundance of a protein in a given gel spot was determined by the corresponding spectral count number (redundant peptides and non-redundant peptides) as previously described (Liu et al. 2004; Roth et al. 2006; Vogel and Marcotte 2008).
Redundant peptides were removed.
Proteins identified from redundant peptides that matched to different proteins entirely were removed.
We predicted 8'085 (redundant) peptides and 14.9% of them were mapped onto the KEGG biochemical pathways.
We predicted 8'085 (redundant) peptides corresponding to approximately 4'000 complete or partial protein-coding genes using a gene model designed for Augustus software [19].
For proteins identified from redundant peptides, we retained those proteins in our catalogue only if the redundant proteins matched to a closely related isoform of the same protein in the database.
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The drying gas temperature was 300°C, and normalized collision energy of 1.3 V was employed for collision-induced dissociation along with a dynamic exclusion of 30 s to reduce redundant peptide selection.
The exact formula used to calculate relative protein abundance is as follows: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0007906.e002.PNG" class= inline-graphic"/> where Aprotein (protein abundance) is expressed as a ratio of the total redundant peptide identifications per protein to the total number of scans identified in the experiment.
The redundant peptide was not extracted from the SWCNT-MA suspension.
The final SWCNT-MA suspension (with the redundant peptide) was stocked for administration to the culture cells.
The same peptide, and therefore protein(s), may be detected in replicate experiments and the redundant peptide count provided yields an estimate of the relative levels of detection.
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