Possible applications include allowing developers to set their redundant file backups (which rarely need to be quickly accessed) to 'offline', where they can be stored at only a fraction of the normal cost.
After trimming the 3' adaptor sequence, all sequences ranging in length from 18 26 nt were recorded in a non-redundant file along with copy number.
We assembled the transcriptomes of Cyclorana and of the developmental stages of Silurana using different k-mer lengths (k = 23, 31, 51), merged the contig files and constructed a non-redundant file using the program CD-HIT [ 107, 108].
LEAPdb contains also roughly 1790 non-accessible entries, classified as "not wanted" either because they are redundant LEAP files or because they are wrongly annotated.
Non-redundant bed files of exon sequences from the zebrafish zv9.71 assembly were intersected with reads using bedtools [ 44].
In order to be able to directly compare our data to that of [ 22], we downloaded their supplementary data and created a redundant sequence file to use as input to the UEA plant sRNA toolkit.
For G. armourianum, 5,723 Class I, 12,033 Class II (Additional file 8), 4,648 Class III (Additional file 2) and 2,425 redundant SNPs (Additional file 9) were obtained for a total of 24,829 SNPs.
A total of 6,663 SNPs were retained for G. mustelinum, 822 in Class I, 4,085 in Class II (Additional file 6), 1,107 in Class III (Additional file 2) and 592 redundant SNPs (Additional file 7).
The web server input of NGSmirPlant is pre-processed FASTA format where all the unique sequence tags aggregated with a number behind the name to represent the expression level and cleaned into a non-redundant FASTA file to reduce the input size.
