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Fig. 1 Multiple sequence alignment of OYE2p with OYE family enzymes with reductive activities toward citral.
Characterization of the purified enzyme showed that KmGRE2 harbored strong NADPH-dependent reductive activities toward at least 10 aldehyde substrates (Tables 2).
The Soxhlet extract exhibited the strongest radical scavenging and reductive activities as compared to the other extracts, most probably due to the highest concentration of phenolics, especially rutin.
In contrast, significant differences were observed in their reductive activities: EhPDICC/SS retained about 60%, whilst EhPDISS/CC roughly retained 20%.
The disulfide reductase systems are integrated with the cytoprotective Nrf2 pathway, thereby allowing cells to match reductive activities with varying stress conditions.
Given that the enzyme AKR1C4 has both dehydrogenating and reductive activities in the steroidogenetic pathway, a missense variation in the gene may predispose to manic/hypomanic irritability by altering the relationship between progesterone and allopregnanolone.
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Additionally to complex formations, these compounds can exhibit reductive activity towards redox-active metals changing the redox state of metals and therefore their speciation.
Brazilian red propolis extract with high concentrations of prenylated benzophenones has been evaluated in macrophages infected with L. amazonensis and demonstrated that a concentration of 25 μg/mL is capable of increasing the reductive activity of MTT being active against these intracellular parasites present in macrophages [76].
This reductive activity eliminates ROS generated either directly or indirectly by metals.
An expanding array of Cr6+ reductases allows the selection of enzymes with higher reductive activity, which genetic and/or protein engineering may further enhance their efficiencies.
The calculated electron affinity energy of the investigated solvents and additives reveals that 3SF shows the highest reductive activity, which is further confirmed by the cyclic voltammetry test.
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