Sentence examples for reduction of the interchain from inspiring English sources
Therefore, the introduction of the designed interchain disulfide bond enhanced the thermostability of FabΔSS and mitigated the destabilization caused by partial reduction of the interchain disulfide bond at the C-terminal region, which occurs in site-specific modification such as PEGylation.
Thiol conjugates are produced by partial reduction of the interchain disulfides, followed by conjugation with a drug-linker, resulting in a heterogeneous mix of molecules that differ with respect to the site of conjugation and the number of drugs per antibody.
On the other hand, none of the other four constructs was prone to autocatalysis either in the presence or in the absence of 5 mM DTT. DTT was added to ensure reduction of the interchain disulfide bond.
to affect reduction of the interchain disulfide.
Reduction of PDI drives isomerase-catalyzed reduction of the interchain disulfides of insulin with consequent accumulation of the isolated weakly soluble isolated B-chain.
A typical IgG has far too many lysine residues for such an approach to be possible, but reduction of interchain disulfide bonds followed by conjugation to the resulting eight cysteine residues is more tractable (an IgG also has 12 intrachain disulfide bonds, but selective reduction of the four interchain disulfide bonds while keeping the IgG intact is straightforward).
Borrowing on the procedure developed to prepare THIOMAB conjugates, complete reduction of the antibody (including interchain disulfide bonds) followed by reoxidation and conjugation with a maleimide-linked MMAE yielded a conjugate with a DAR of 1.3.
The decrease of gelation efficiency for the modified alginate samples reflected the reduction of ionic interchain bonds as a result of the unavailability or non-accessibility of G units on the alginate backbone, due to the chemical modification and steric effect of the CD molecule for alg-CD.
ER-retained P23H rod opsin is subject to retrotranslocation and ER-associated degradation (ERAD), a process that requires the reduction of any interchain or intrachain disulphide bonds (16).
Although this increase was much lower than the one observed in Rho(P23H -GFP, the effect of DTT might reflect a reduction of P23H -GFP inthechain bonds as a reffectof C185A or C185S substitutions.
ADCs are commonly prepared by chemical conjugation of small molecule cytotoxic anti-cancer drugs to antibodies through either lysine side chains or cysteine thiols generated by the reduction of interchain disulfide bonds.
