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Interestingly, this modest reduction of Rot reduced expression of Protein A as much as 10 fold.
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Notably, the clpP mutant strain contained wild-type levels of Rot suggesting that the reduction in rot transcription observed in clpX and clpP mutant strains is not reflected in the cellular Rot level, and hence that the ClpP independent effect of ClpX in controlling Rot synthesis is achieved mainly at the post-transcriptional level.
After confirming that ClpX is required for full expression of Rot, we examined whether the 2 3 fold reduction of the Rot level can explain the dramatically reduced Protein A level of the clpX mutant [20].
Three different mechanisms have been proposed for the reduction of Fe3+ in wood-decaying brown rot basidiomycetes: (i) iron-reducing enzymes, such as cellobiose dehydrogenase (17), (ii) low-molecular-weight glycopeptides (18), and (iii) secondary metabolites, including hydroquinones, such as 2,5-dimethoxyhydroquinone (2,5-DMHQ), and catechols, such as 4,5-dimethoxycatechols (DMC) (16, 19).
The building smelled of rot.
But pockets of rot can wreak havoc.
The overall figures hide lots of rot.
This is a lot of rot.
But there is plenty of rot, too.Start with takeovers.
The woman is obsessed with its smell of rot.
That's the first sign of rot.
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