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The column "reduction" shows the percentage of reduction of function evaluations compared with DE.
Considering the reduction of function evaluations, DE-NNC and DE-EC were not much different.
Reduction of function of the demethylases potently suppresses longevity and UPRmt induction, while gain of function is sufficient to extend lifespan in a UPRmt-dependent manner.
These results show that reduction of function of any one of these genes is capable of converting the weakly hypoxia resistant e1368 animals to strongly resistant ones.
The specific reduction of function mutants of daf-2, an insulin/insulin like growth factor receptor, has previously been reported to promote anoxia resistance in C. elegans [27].
The loss (or reduction) of function in PfCRT could be compensated through increased expression of some other transporters such as PfVP2 and/or changes in CN of pfmdr1.
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In order to functionally characterize BdCAD1 and BdCOMT4, reduction-of-function mutants were developed and assayed for changes in secondary cell wall composition.
We have previously shown that reduction-of-function mutations in the daf-2 gene confer powerful protection from hypoxic injury in the nematode Caenorhabditis elegans [1].
The die genes are candidates to promote daf-2-regulated hypoxic sensitivity; thus, die gene reduction-of-function might produce hypoxia resistance.
These new data suggest that a positive feedback loop may exist that enhances the reduction-of-function phenotype of e1370 compared to m596 and e1368 by reducing the levels of akt-2 mRNA.
To confirm whether the retroviral insertion indeed resulted in a loss- or reduction-of-function allele, we performed quantitative RT-PCR (Q-RT-PCR) using primers designed to amplify the 3' UTR of Sulf1.
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