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The results show that despite pronounced reduction in sequence complexity, pure symmetry in the design of a foldable, thermostable β-trefoil fold is possible.
Following training the impaired PD group showed significantly greater reduction in sequence initiation and completion time and in error rate for IR conditions compared to the unimpaired PD and control groups.
This reduction in sequence redundancy resulted in a unigene dataset of 105,481 (Table 1).
A general reduction in sequence complexity across all size classes of viral siRNAs was observed in both the rdr1 and rdr6 mutant libraries, with a much more profound reduction found for the most part in the rdr1 library (Fig. 4A; red and blue traces).
The observed reduction in sequence variation might be a consequence of purifying selection [ 43].
This procedure resulted in 57 % reduction in sequence length on average.
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Specifically, there was a 10.6-fold reduction in sequencing costs with our methodology.
Recent improvements in the annotation of the zebrafish genome coupled to a reduction in sequencing costs prompted the development of whole genome and RNA sequencing approaches for gene discovery.
We tested the effect of a ten-fold reduction in sequencing error (from 1% to 0.1%; Fig. 5B).
We have sought to project the effect of this cost reduction on phenotype sequencing in Fig. 5B, which considers the effect of a two-fold reduction in sequencing cost.
In recent years, however, advances in sequencing technologies have allowed drastic reduction in sequencing costs.
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