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This assay is based on the reduction in expression of a luciferase reporter gene that was co-expressed with WT or each of Bax mutants.
This observation is similar to the differentiation-associated reduction in expression of components of the transcriptional machinery that are essential for the activation of myogenic loci37.
Loss of dco in the wing disc results in a dramatic reduction in expression of the caspase inhibitor DIAP1 and a concomitant activation of caspases.
12/15LO Xinhibition also reduced hypobaric hypoxia-mediated upregulation of hippocampal HIF-1α protein expression along with reduction in expression of inflammatory genes.
Whereas, VCD caused a reduction in expression of AMH (d4 d8), it followed previously reported initial disruptions in Kit signaling induced by VCD (d2).
This may be partly due to about 50% reduction in expression of the myosin light chain (MLC) and CPI-17 (Huh et al. 2011).
Himeda points out that while they achieved a 50percentt reduction in expression of the FSHD disease gene, we still don't know what the effects are on all the other genes in a cell.
As expected, hog1 mutation caused significant reduction in expression of all these genes.
Additionally, reduction in expression of nucleoporin 153 by siRNA targeting reduced ERK1/2 nuclear activity in cancer cells.
By contrast, the higher affinity DO11.10 system had less of a reduction in Th-POK and a larger reduction in expression of Runx3, GATA3 and TOX.
Injection of MOXMeis3 leads to a reduction in expression of Hoxd1, Hoxb4, and Hoxc6 in mesoderm and ectoderm during gastrulation, and to severe patterning defects.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com