Obtaining early passage mESCs and reducing the manipulations required to generate the desired construct can make germline transmission more likely.
It is likely that reducing the manipulations between sedation and induction, a lower dose of romifidine could be used in some animals; however, due to the frantic temperament of most colts, an intravenous catheter could not have been placed before sedation in the majority of the animals.
No count of attached ticks was made to reduce the manipulation of animals and ticks, and dogs were further fitted with Elizabethan collars during the tick infestation period to avoid the removal of the ticks through grooming.
Merin 1994 seeks to reduce the manipulations to a sequential combination of so-called elementary social acts: claim, concession, denial, and retraction.
Simplifying the practical moments and reducing the physical manipulations of the cell cultures are also issues that are important to deal with at an early stage of protocol development and cross-disciplinary collaborations are well equipped to address these challenges.
The results also indicate that manipulation has a significant impact on call auction prices, with some algorithm designs more effective than others at reducing the impact of manipulation.
By reducing the amount of manipulation we observed an increase in the total number of viable OPCs, and minimized cell death.
Therefore, the IOL was not determined to be implanted in our cases, which might reduce the surgery manipulation and the risks of complications.
INAA is well known as reference analytical technique because all the experimental steps are totally traceable and there is absence of physical-chemical sample manipulation reducing the positive and/or negative artifacts formation [7, 9, 20, 21].
Using ultrasound, full reproducible food processes can now be completed in seconds or minutes with high reproducibility, reducing the processing cost, simplifying manipulation and work-up, giving higher purity of the final product, eliminating post-treatment of waste water and consuming only a fraction of the time and energy normally needed for conventional processes.
