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Fig. S1 (See Additional File 1: Fig. S1) shows marked degradation of RNA in the sample incubated at 37°C, supporting the efficacy of this treatment in preventing or reducing potential contamination from adjacent tissues during dissection.
However excluding all uninterrupted open reading frames (ORFs) of at least 30 codons from the intergenic DNA, and thus reducing potential contamination from recently pseudogenized genes, has very little impact on the ability of intergenic composition to predict AAC (supplementary fig. S2, Supplementary Material online).
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When the cost was not prohibitive and it was offered, sashimi (a slice of fish with no rice or wasabi) was purchased instead of nigiri sushi to reduce potential contamination due to handling.
Randomization was carried out at the level of the preschool to reduce potential contamination from parents at the same preschool sharing intervention information or resources.
The team spent significant time in the training session practicing nonjudgmental questions and probes with the facilitators to reduce potential contamination of the findings.
However, we think that the participants can be blinded since they will not be aware of the precise type of mobilisations received and to reduce potential contamination bias, the two programs will be given in different clinics.
To reduce potential contamination from co-purified kinases in these assays, we conducted in vitro kinase assays using recombinant full-length GST-tagged Drp1, Mfn1 and OPA1 combined with soluble recombinant full-length LRRK2 variants in the absence of Dynabead complexes.
To reduce potential contamination, we used a cluster randomisation design in which we randomised sites rather than individual patients to either manual office blood pressure with continuing use of manual sphygmomanometry (control group) or management of the study patients with automated office blood pressure (intervention group) using the BpTRU device (BpTRU Medical Devices, Coquitlam, BC, Canada).
Proper site selection for the location of domestic water wells and proper well construction can reduce potential nitrate contamination of drinking water source.
In addition, hand dug wells for domestic use should be sited above 30 m from cocoa farms to reduce potential pesticide contamination as most of the water samples analysed within distances 0-30 m were found to be contaminated with pesticide residues.
To reduce potential for contamination of PCR reactions, amplifications were carried out in a PCR hood using dedicated, UV resistant pipettors with factory sterilized barrier tips.
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CEO of Professional Science Editing for Scientists @ prosciediting.com