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The unique design of the device increases the surface area-to-volume ratio and offers the possibility of reducing equilibration times and higher assay throughput.
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First, the pallet, placed in an air tight polyethylene casing, was flushed with a N2-enriched gas mixture at the maximum flow rate that the generator could deliver to reduce the equilibration time.
However, the device presented here reduces the equilibration time by orders of magnitude, yields concentrations below 0.5% oxygen, and permits very complex oxygen profiles across the same well.
Also, we show that the main limitation to reducing the full equilibration time is related to instrument design issues; a novel approach to overcome these instrumental issues is described.
Long equilibration times of several hours were observed.
Initial solution equilibration tests and laboratory core incubation experiments suggest that equilibration times for probes in sediments are approximately 24 48 h.
We show that equilibration times of about 40 ns are required to yield convergence.
Additional experiments using equilibration times longer than 30 min were also carried out.
Fib does not significantly contribute to the corona, even at longer equilibration times.
Nanospheres, combined with the streak-seeding method, produced single 5F2 Fab crystals in shorter equilibration times.
In contrast, crystals did not grow in the control droplets with equilibration times of one and four days.
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