All variants had reduced specificity for DHB.
In conclusion, use of the 2 immunomodulators did not improve sensitivity of the QFT-GIT in TB patients and reduced specificity in low-risk health care workers.
Endoscopy as reference standard 994 Sensitivity/specificity of single reading, 76/91%; simultaneous dual reading, 76/86% Dual reading led to an increased number of false positives which reduced specificity.
This reduced specificity is not a serious problem.
Population specific ROC analysis could not be performed to determine the cost in terms of reduced specificity.
The "cost" of false positives (i.e. reduced specificity), although more than twice that of conservative algorithms, was below 1.5% and might be acceptable for rare variant discovery studies (Fig. 3B).
The combination of methods had higher sensitivity (88.0% vs 67.4%) but reduced specificity (88.0% vs 69.5%).
Reduced specificity of concentrated microscopy compared with direct smears has been reported previously [ 10, 11].
This study only used a tissue-like pattern to diagnose consolidation which may have reduced specificity.
As expected running MegaBLAST without soft masking also leads to significantly reduced specificity (data not shown).
Expression of R133C protein from an integrated transgene in embryonic stem cells also revealed reduced specificity for methylated DNA (24).
