These issues effectively reduce sample throughput by increasing the time needed to collect individual spectra.
The advantages of ICW over WB are its high-throughput capacity, improved precision, and reduced sample preparation requirements.
This direct, ambient ionization approach offers reduced sample preparation and analysis times compared to previous GC and LC techniques, enabling high throughput analyses.
As a result, the identification and quantification of the studied compounds will be much faster, the total analysis cost will be reduced and sample throughput will be significantly increased.
A useful repertoire of proteomics technologies is currently available for disease-related applications, though further technological innovations would be beneficial to increase sensitivity, reduce sample size requirements, increase throughput, and more effectively uncover various types of protein alterations, such as post-translational modifications.
This approach is advantageous over using gas chromatography for TCE and HPLC for the surfactants because the use of a single analytical instrument reduces sample preparation and analysis times, which increases sample throughput.
Recently, cell chips have emerged as a promising technology for drug discovery/delivery, and their miniaturization and flow-through operation significantly reduce sample consumption while dramatically improving the throughput, reliability, resolution and sensitivity.
Other mass spectrometry-based techniques could be used, such as multiple reaction monitoring (MRM) and selected reaction monitoring (SRM) LC-MS/MS, which allows focused study on only m/z ratios of interest, reducing sample processing time and greatly increasing throughput.
Alternative high-throughput strategies focus on reducing sample complexity by using methylation profiling, either through genomic DNA restriction by methyl-sensitive endonucleases, bisulfite conversion strategies or immuno-precipitation using methyl-binding proteins or antibodies against methylcytosine-containing sites [ 26, 27].
Selective pressurized liquid extraction (SPLE) is an analytical technique that builds upon PLE by incorporating matrix-compound (i.e. interference) retainers into the extraction step, thereby reducing sample-preparation steps and increasing sample throughput.
