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We next reduced sample variability using the voomWithQualityWeights function of the limma R package prior to DEG discovery.
That said, this restriction does limit the generalizability of the results obtained from the reduced sample.
In Table 2 resulting drop-out rates and reduced sample sizes are stated.
The results are consistent in both the larger sample containing pair-mates and the reduced sample.
This reduced sample sizes for the months of moult (June-October).
The advantages of ICW over WB are its high-throughput capacity, improved precision, and reduced sample preparation requirements.
8μl of diluted and reduced sample was loaded onto the gel for each sample.
However, low numbers reduced sample size in two lines (17, 95) in generations one and two.
Such variations potentially affected the overall range of signals between arrays and therefore reduced sample comparability.
This simplified lysis step reduced sample handling and loss, which would impact on ChIP efficiencies.
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The non-reduced sample was then reduced following the procedure using iodoacetamide and DTT described above and analyzed again.
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