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We measure the integrated performance of the studied protocols as follows, (mathcal {P}^{text {ip}} = frac {mathcal {P}^{mathrm {{th}(bps)}}}{mathcal {P}^{text {md}}(s) times mathcal {P}^{text {oh}}}), which quantifies the cost compensation for the increased throughput and reduced medium access delay performances.
A) HT29 and SW480 cells were grown in full supplemented or reduced medium to induce starvation.
The fully reduced medium was inoculated with 10 mL of a R. albus 7 (ATCC 21270) culture grown for 24 h on the same medium.
Foetal calf serum (FCS), horse serum (HS), Dulbecco's modified Eagle's medium (DMEM), OPTI-MEM1 reduced medium and lipofectamine were purchased from Life Technologies (Paisley, Scotland).
Subsequently, cells were subjected to growth factor reduced medium with or without AEA and different endocannabinoid receptor agonists and antagonists as indicated.
The next day, cells were transduced with 0, 30 30, 100, 300, and 1000 pfu/cell of dcn-pxc1c-1 or RAdlacZ in reduced medium containing no FBS.
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The reduced film was used as reducing medium and host for the formation of Au nanoparticles [17].
The BBC Trust said that it agrees "in principle" with plans to reduce medium wave transmission for local radio where there is duplicate FM coverage.
Na2S·9H2O (0.50 g/L) was used to reduce medium and the final pH of the basal medium was adjusted to 7.2.
However, further experiments are needed to determine how to prevent multi-stage failure of reducing medium volume.
Briefly, 60 ml bottles were filled with 2 g dry weight sediment and 50 ml methanogenic medium (Holliger et al. 1993), sulphate reducing medium (Stams et al. 1993) or nitrate reducing medium (Evans et al. 1991) was added.
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