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The covering of these scales with nail polish muffled the sounds and significantly reduced mating success in O. nubilalis, showing the importance of ultrasound signaling in mating.
Procedures used to induce mating, meiosis, and preparation and plating of ascospores were as described [52], [57] with several minor changes to help address the fact that the atf1Δ and spc1Δ mutants are sensitive to stress conditions and have reduced mating efficiency and spore viability [12], [26].
In contrast, males with no gift experienced a significantly reduced mating success.
miR-124 mutant males showed reduced mating success when paired with female flies, and elicited courtship by normal males.
Infected males were found to have reduced mating vigor, as well as decreased sperm production and sperm competitive ability [ 39].
These examples suggest that the reduced mating success of unmated females' sons might not be as high as expected.
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For example, pheromone traps used to attract and kill pest insects might long retain their effectiveness because reduced responses to these pheromones could reduce mating success (Witzgall et al. 2010).
Conflicts can, for instance, revolve around the frequency of mating, with males expected to persistently try to increase the number of matings, while females will try to reduce mating rate [ 9, 10].
The worms also reduce mating success and survival in male flies.
Based on our mating assays, we have demonstrated that deletion of the SSN8 gene results in enhanced mating filamentation, whereas artificially elevating the SSN8 transcript level reduces mating filamentation, suggesting that C. neoformans Ssn8 plays a negative role in heterothallic a α mating process.
Finally, RNAi with the MMR-B3 gene significantly reduces mating behavior.
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