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Introduction of G18A and U54C mutations, which disrupts the interaction between the D and TΨC loops, reduced label transfer efficiency.
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There was no requirement for it to do so because of reduced labelling requirements for food produced on-site.
In special cases, reduced labeling degree of the tracer substrate can increase the precision of flux analysis.
24-hr exposure to MβCD visibly reduced labeling intensity.
Tissue incubated with aldosterone and the antibody MA1-620 had the appearance of reduced labeling.
One month of IL-1Ra treatment significantly reduced labeling of GK islet alterations, as shown for both VWF and fibronectin (−53% and −69%, respectively).
Standard immunocytochemistry of control brains (n = 2) produced the typical pattern of strong neuroglian labeling only in the SZ and in glomeruli (Fig. 13A), while treated brains (n = 3) displayed significantly reduced labeling, especially in the SZ (Fig. 13B).
Indeed, we observed that the immunolabeling of Brp puncta is reduced, suggesting that the amount of Brp protein per puncta is decreased.[51] The reduced labeling of Brp and Syb in the presynaptic motor neuron of wnt2 mutants is not likely due to changes in transcriptional mechanisms.
Labeling one day earlier (E4.5) marks a similar distribution of cells but with reduced labeling.
The specificity of TPC2 antibody labeling was verified by preblocking with the immunogenic peptide, which reduced labeling by 87.4% ± 0.4%.
However, these attempts also reduced labeling specificity in parallel to increasing signal intensity, as determined in control knockout samples.
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