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The medium components as well as the highly reduced conditions of the T. saccharolyticum fermentation broth may contribute to this difference.
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NR was also unaffected by the addition of resazurin as a redox indicator for reducing conditions of the ruminal media.
The pH of the reaction mixture can significantly alter the reducing conditions of the environment playing a major role in the synthetic procedure.
In particular, protein conformational folding and structural stability of the newly-synthesized intrabody within the cell is affected by reducing conditions of the intracellular environment.
A rapid decrease in the catalytic activity under dry-reforming conditions is due to excessive carbon deposition under more highly reducing conditions of dry-reforming compared to the other reforming conditions.
Western blot analysis under reducing conditions of periplasm extracts showed that the migration pattern of secreted L36N and 2H1N was consistent with the molecular weight calculated from the polypeptide sequences (Fig. 4a).
In the reducing conditions of these peatlands zinc sulfides occurred as framboidal aggregates of sphalerite and polytypic wurtzite (2nH, n ⩾ 2) nanocrystallites associated with bacterial cells and organic matter.
Chalcophiles formed as the crust solidified under the reducing conditions of the early Earth's atmosphere.
Even in the reducing conditions of SDS-PAGE, PknJ forms stable dimer, which is also shown by variants PknJ-KD-K43A and PknJ-KD-H78A.
Moreover, these effects were independent of the reducing conditions of the reactions or the expression of the N-terminal inserts, and they involved a specific interaction between 3R and 4R tau isoforms.
Coomassie stained gels of the pellets containing polymerized tau showed that assembly reactions of 3R0N and 4R0N tau isoform mixtures again contained less aggregated tau than the single isoform reactions as observed under the reducing conditions of the cell.
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