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The use of bootstrap to estimate confidence intervals may have reduced any effect of overfitting.
Such misclassifications are likely to have reduced any effect towards a null hypothesis.
It is unlikely that reliever use in the asthmatic children significantly reduced any effect size seen, since reliever use was generally very low, and the variable used for any reliever use was only retained in one model in the multivariate analysis.
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In order to reduce any effect from phylogenetic distance of the host roots, and to compare a similar number of samples from wild and cultivated species/cultivars, we also made a similar comparison limited to the AA genome species (Additional file 3: Figure S3).
We utilized a sparse-sampling experimental design, in which tasks were performed during the silences between image acquisitions to prevent scanner noise from interfering with the auditory stimuli and to reduce any effect of movement [52].
Drosophila were transferred to new food vials once every 7 days without CO2 anaesthesia to reduce any effect on lifespan.
We also sampled 50 proteomes equally from cells and dsDNA viruses to reduce any effect of the large number of bacterial proteomes in our dataset.
At each ILD, the stimulus was repeated 20 times, and in each block, ILD stimuli were alternated so that a larger contralateral level was followed by a larger ipsilateral level to reduce any effect of adaptation.
The presence or impact of such bias is unknown but in this and future trials the inclusion of a similarly highly motivated control may help reduce any effect of such bias.
As described in the discussion, we chose this reference day for consistency and to reduce any effect on the measured performance of the reference day from longer admissions in which care was received at the weekend.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com