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Formulations reduced aggregation of native Fab and IgG relative to vehicle up to 50% and enhanced transepithelial permeation rate up to 2.8-fold.
Previously, we optimized PAL from R. toruloides, and in this case we reduced aggregation of the A. variabilis PAL by mutating two surface cysteine residues (C503 and C565) to serines.
The fusion to MBP reduced aggregation of DivIVA and this made it possible to test membrane binding using a simple sedimentation protocol.
Interestingly, the consequence of this increased solubility differs depending on the mutant; whereas UGGT1 improved secretion of the non-native form of NHK, it reduced aggregation of ATZ and retained it in the ER in a soluble, misfolded form (Ferris et al., 2013).
The decrease in ataxin-2 reduced aggregation of TDP-43, markedly increased survival and improved motor function.
All techniques indicate a reduced aggregation of PTN+Me3 in DMSO relative to water and a marked de-aggregation of PTN+Me3 in water following addition of bile-acid anions such as deoxycholate and ursodeoxycholate.
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Flavonoids may prevent or slow the progression of AD by interfering with the generation and polymerization of amyloid-β peptides into neurotoxic oligomeric aggregates and also by reducing aggregation of tau proteins [ 50].
The rule-set automatically reduces aggregation of particles of same composition to a 1-d problem.
Simple conjugation to poly ethyleneglycol) can increase half-life, decrease renal clearance, improve stability and reduce aggregation of antibody fragments without compromising their antigen binding.
Fusion proteins, cryS96 and crySI, were found to reduce aggregation of alcohol dehydrogenase and insulin, which demonstrates that ELP fusion did not diminish chaperone activity.
And HKK-BTZ1 was modified by introducing alkoxy group of TPA unit, because the bulky alkoxy group is a strong donating group for the more red shift and for reducing aggregation of dyes in TiO2 film.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com