W430A had strongly reduced affinity for tetramethylammonium.
By contrast, the U state has a greatly reduced affinity for both metals under these conditions [6], [7], [24], [25].
TEM-33, also called inhibitor resistant TEM 5 or IRT-5, has a reduced affinity for β-lactamase inhibitors.
This could be explained if the Q169 mutants have reduced affinity for the nucleotide that would be incorporated at this position.
Alternatively, isolated CbfA-CTD may have a reduced affinity for its target promoter sequences and may be unable to displace endogenous CbfA.
The mutation-specific primers (Table 1) were designed to preferentially anneal with the targeted mutation nucleotide(s), thus having reduced affinity for wildtype sequences.
The R69A MtBPL has similar affinity for biotin as that of wild type in contrast to R118G EcBirA which exhibited reduced affinity for biotin.
Acetylated histones have a reduced affinity for chromatin, allowing chromosomal unfolding that can potentially modulate the expression of particular genes, including fetal forms of adult genes.
In contrast, in the LDD alone, the HOIPLDD C930A has a greatly reduced affinity for ubiquitinTAMRA.
The hexa mutant showed reduced affinity for Isu1 in vitro, consistent with this region participating in a binding interaction.
This mutated protein would be expected to be deleterious, having reduced affinity for GC-box binding sites in promoter regions.
