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However, it was shown that a truncated chlorophyll antenna mutant of the green alga Chlamydomonas reinhardtii exhibited reduced absorbance of light by the first layers of cells and alleviated photoinhibition, resulting in high biomass production (Polle et al. [2003]).
Particularly, people pay close attention to near-infrared (NIR) QDs for visible in vivo tissue imaging due to their reduced absorbance and scattering in biological tissues within the NIR region, as well as the strong penetration in human tissues.
Furthermore, it had been shown by electron microscopy that chloroplasts isolated in media containing 5 mM MgCl2 retained stacked thylakoids, while chloroplasts isolated in low salt media lost their grana structure and had a reduced absorbance at 580 nm [9], [10].
However, a majority of itprsv35/ug3 mutant larvae had no or very little food in their gut and thus exhibit reduced absorbance values indicating that they were unable to feed normally (Figure 2A, B, 60 hrs after egg laying (AEL) and Figure 2C, D, 108 hrs AEL).
Linear regression analysis was used to compute the concentration that reduced absorbance by 50% (IC50).
The scavenging activity was defined as the percentage of the reduced absorbance caused by treating DPPH mixture with samples.
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One unit of SOD was defined as the amount of enzyme necessary to inhibit formation of blue formazan to reduce absorbance to 50% of blank's.
Scavenging% was plotted as a function of the mass of protein contained in the sample in order to obtain the mass of protein necessary to reduce absorbance by 50% (IC50).
DPPH is often used to assess antioxidant activity by determining the change in absorbance at 517 nm via UV vis spectrophotometry; the solution color turns from deep purple to light yellow upon free radical quenching, thus reducing absorbance at 517 nm.
It should, therefore, be possible to reduce the inducer concentration ten-fold, reduce absorbance time of the induction moment and the concentrations of yeast extract, glucose, and glycerol to the minimum levels of the ranges tested, keeping rPly protein expression at high levels (250 mg/L, which corresponded to 25-28% of the total E. coli protein extract) and reducing process costs.
Each absorbance cycle is again fully reproducible, whilst the gradual build-up in absorbance over the same time scale as part a is reduced (maximum absorbance at t = 20 min is ∼0.25 in part b, compared to ∼0.7 in part a). Figure 5c shows the profile obtained when the scan range is +/ 1.75 V, and the scan rate reduced to 10 mV/s in parallel.
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