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This should reduce the assay time, which is a necessary requirement for real life applications.
We will investigate the effects of herringbone geometries on mixing in the future to reduce the assay run time.
We refined the fluorescent bead-based immunoassay to reduce the assay turnaround time from 4.5 hours to 2 hours, rendering it suitable for routine clinical use.
The use of these rapid methods could therefore reduce the assay time from a few days to 24 h including enrichment time.
To further reduce the assay time and avoid contamination, a one-step multitarget RT-PCR was performed in one tube rather than multiplex two-step RT-PCRs in separate tubes [ 12] would be preferred.
Firstly, direct labeling of SH2 domains or the GST tag with oligonucleotides would reduce the assay complexity and could prevent issues stemming from insufficient GST:anti-GST antibody binding.
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This unusual design drastically reduces the assay background signal.
The use of magnetic nanoparticles increases the volume-to-surface ratio reducing the assay time to 30 min.
Furthermore, since the assay has a Q10 for peroxyl radical-scavenging of about 3, elevation of the assay temperature from the standard 37 to 47°C significantly reduced the assay times.
Using higher lymphocyte count in smaller volume of culture media increased detection and reduced the assay duration to ∼30 hrs.
In conclusion, the findings show that simultaneous determination of at least two antigens, LAM and BCG and application of the micro-ALS method improves diagnostic accuracy of the ALS assay and reduces the assay time considerably.
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