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We tested this hypothesis using RNA interference to reduce steady state levels of COMP mRNA.
In contrast, the integrants containing GA and GB shRNAs that target globular domain sequences did not reduce steady state WT- or MT-COMP mRNA or protein levels (Fig. 2 and data not shown).
Although phenotypes associated with each treatment were not reported, mixing spores with dsRNA was shown to reduce steady state mRNA levels after a 24 hr incubation period.
Throughout these results, we report therapeutic biochemical efficacy in terms of an IC50, defined as the therapeutic concentration required to reduce steady EGFR phosphorylation to half its value in the absence of therapeutic.
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In addition, they reduce steady-state mRNA levels of enzymes involved in PAPS synthesis.
As expected, inhibition of either CO or H2 uptake was predicted to reduce steady-state biomass production throughout the column (Fig. 8).
These GFPs all displayed progenitor-like fluorescence spectra, temperature-sensitive folding, a reduced structural stability and, for the least stable variants, a reduced steady state abundance.
In summary, reduced steady state serum concentrations of progesterone from a CIDR device resulted in continued growth of persistent follicles in cows and heifers compared to a non-manipulated CIDR device.
Moreover, we show that reducing steady state levels of COMP mRNA alleviates intracellular retention of other extracellular matrix proteins associated with the pseudoachondroplasia cellular pathology.
Second, the half-life of the ubiquitinated intermediates may be quite short, further reducing steady state values of the degradation intermediates.
We found HRK siRNA treatment significantly reduced steady state mRNA pools of HRK in HCSF treated with C6 ceramide for 6 or 12 hours compared to siNTC transfected cells that had been treated with ceramide (Figure 5A).
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