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Four key technologies were developed to support this system: drill bit development for varied substrates, sample acquisition mechanisms to acquire cores at depth, material transport technologies to move waste material up the hole, and sample reduction technologies, studying the means to efficiently reduce samples into uniform particle sizes.
NaBH4 (50 mM) (Sigma-Aldrich) was used to reduce samples as indicated.
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The obtained results have shown to be useful to increase selectivity and reduce sample handling.
Colony-based assays are simple and thus can reduce sample preparation time.
The ion implantation current density was maintained below 2 μAcm−2 to reduce sample heating.
Field sampling was performed by a single surveyor (AZ) to reduce sampler bias.
In addition, extracts may require pre-fractionation in order to reduce sample complexity.
To reduce sampling variance, the results were obtained by averaging 20 Monte Carlo trials.
Proteome fractionation with anion exchange chromatography was used to reduce sample complexity and increase quantitative coverage of protein expression.
These improvements have allowed us to reduce sample input.
This can reduce sample size requirements and thus study costs.
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