Samples were washed in phosphate-buffered saline (PBS), reduced, passed through a filter, and placed in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 0.1% 10 mg ml−1 gentamycin (Invitrogen, Grand Island, NY, USA).
The exchange of cast iron brake blocks by composite ones is a well-known low-cost measure to reduce pass-by noise.
A bill to allow the government to negotiate directly with drug companies to reduce prices passed through the House but stalled in the Senate.
The league took over effective control in these new talks, and Hornets General Manager Dell Demps, who made the doomed deal with the Lakers, has been reduced to passing proposals back and forth between the Clippers and N.B.A. officials.
They played so deep that United were never able to get behind the full-backs and were reduced to passing the ball across and in front of the line of nine, sometimes ten, blue shirts in front of them.
This paper describes two configurations of feedforward adaptive active-noise control (ANC) technique for reducing blade passing noise in centrifugal fans.
Furthermore, the waste gas ingredients, including H2S, NH3, greenhouse gas (N2O) and microbial aerosols, were remarkably reduced after passing through the VFCW.
The first reduction of pathogens was reported by USEPA (1999) where pathogens are reduced by passing biological solid waste through the worm intestine.
Moreover, transfection of mouse E-cadherin cDNA into HP cells reduced both passing activity through the membrane of the invasion chamber and peritoneal dissemination in nude mice to levels similar to that of LP cells.
The inference power of this systematic analysis is substantially reduced when passing from E.coli to S.cerevisiae.
