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Biochemical analysis of each ETC complex showed that complexes I to III were reduced in activity (Figure 4) while those of complexes IV and V were increased (Figure 4 D and E) in accordance with previous analyses [12].
Interestingly, reporters carrying the hybrid motif had reduced in activity in the presence of DMI, while those with the canonical C/EBP sequence were increased by DMI.
The enzyme kinetics revealed that the ENPP1 isolated from the liver of asj mice was markedly reduced in activity (Fig. 7C).
Most mutations have been found to cause a reduction in dismutase activity, while some (for example G37R, A89V and D90A) are essentially normal or only slightly reduced in activity (Andersen and Al-Chalabi, 2011).
A promoter mutation showed reduced in vitro activity corresponding to lower mRNA expression in patient samples.
The second cohort had the competitive aspects reduced in the activity.
Likewise, the cIII activity was reduced in mzm1Δ/mzm1 D25N (65% residual activity) and mzm1Δ (50%) mitochondria (Fig. 2C).
Indeed, these Mcl-1 inhibitors exhibited reduced in vitro cell activity in the presence of serum.
(A ) Immunodepletion of ERGIC membrane from L fraction reduces in vitro lipidation activity.
A recent study demonstrated reduced in vitro CYP3A4 activity due to POR A287P (13).
In order to reduce spontaneous activity in DA1 PNs, both antennae were removed with forceps.
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