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Since we found commercial amplification kits to be heavily contaminated with exogenous DNA, and a home-made PCR mixture with chemically synthesized dNTPs to be moderately contaminated, we explored other approaches to further reduce contamination levels.
Technicians at CRESST are currently replacing some of its parts in order to reduce contamination levels within the apparatus itself.
To reduce contamination levels, different solutions are proposed.
Third, as reported earlier, external catalytic gas recombiners can be used to promote H2/O2 recombination and reduce contamination levels in the gas production.
Hadjok et al. (2008) reported UV light (254 nm) combined with H2O2 can reduce contamination levels of human pathogens (Escherichia coli O157 H7, Pectobacterium carotovora, Pseudomonas fluorescens and Salmonella) on or within (internalized) fresh produces but reduction of internalized pathogen was around half of that for surface pathogens.
It should be noted that the ideas above assume an expansion in recycling capacity, which will require associated expansion in collection activities, use of secondary plastic materials and, associated with the latter, better methods for separating the different types of plastic to reduce contamination levels.
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Waste management processes generally represent a significant loss of material, energy and economic resources, so legislation and financial incentives are being implemented to improve the recovery of these valuable resources whilst reducing contamination levels.
A single wiping motion (defined as a 'poor' clean) was less effective than the Nanoclave Cabinet in reducing contamination levels on the display panel but more effective when used to disinfect the probe receptor and earpiece holder.
The spatial and temporal occurrence of pathogenic bacteria was evaluated, as well as the capacity of thermal treatment to reduce contamination to safe levels before consumption.
The variation in lead levels between manufacturers suggests some could reduce contamination (lead is banned from cosmetics in the UK).
Salt alone didn't reduce contamination, but the combination of salting and rinsing reduced salmonella levels by 80 percent.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com