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These pretreatment regimes reduce assay throughput and increase testing costs.
Try to reduce assay time, to get results back to operators quicker, while they still have a chance of remembering the batch concerned.
Recent advances in robotic liquid handling have made it feasible to reduce assay optimization timelines with the application of statistically designed experiments.
Compared with traditional MLA or ELISA methods, the assay takes only half a day and consumes significantly less sample and reagents, which will significantly reduce assay cost and simplify disposal.
The key goals of this development were to reduce assay time while increasing the sensitivity and specificity as compared with a pilot version of a gyrA genotyping DNA microarray.
The same PCR conditions as described for assay development applied, but the reaction volume was reduced to 10 µl and the number of PCR cycles was set to 26 to reduce assay costs and cycler time needed.
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HB101 was prepared in large batches (greater than 20 L) to reduce assay-to-assay variability.
As the purpose of this study was to establish factors associated with VZV susceptibility, reduced assay sensitivity is preferable to reduced specificity [ 10].
Moreover, the main significant advantages over other published works are the simplicity of the sample preparation, reduced assay time and sample loss.
By the combined reverse transcription-asymmetric PCR and cohybridization methods, the DNA chip reduced assay time and was convenient to use.
High-throughput technology played an important role in strain improvement screening, significantly increased throughput and reduced assay volume, efficaciously solve the low-throughput problem encountered in conventional strain screening.
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