The raw intensity of the bleached speckle was normalized involving acquisition bleaching correction, i.e., it was corrected by the background intensity.
To correct for acquisition, bleaching signal intensities were divided by a bleaching factor.
Low laser intensities were used to avoid acquisition bleaching.
The raw data were corrected for background noise and image acquisition bleaching.
For analysis, ROIs were individually reselected in ImageJ at each time point and acquisition bleaching was measured in non-bleached regions.
Data were then corrected for acquisition bleaching and normalised against the pre-bleached value.
To measure acquisition bleaching, intensity measurements were collected from four non-bleached control regions, of the same size as the bleach regions.
Data were corrected for acquisition bleaching and normalised against pre-bleach values.
The illumination technique of ultramicroscopy treats the sample with more care and dramatically reduces bleaching.
Of that increase, the Burt's Bees acquisition, bleach businesses acquired in the 2007 fiscal year and the weaker dollar each added 1.5 percentage points.
