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Linked markers were used to identify the genotype of individual fish for the blond and red loci from the specifically designed crosses so the effect of recessive blond gene on blotched fish could be assessed.
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Open image in new window Fig. 4 a I ds versus V gs characteristics at V ds = 3 V of this FET, the red locus of points represents the curve after annealing (V T = 0 V) and black locus of points represents the curve before annealing (V T = 15 V).
Based on this model, an annealing is processed toward this bilayer MoS2 FET. Figure 4a shows the I ds versus V gs characteristics (V gs ranging from −20 to 40 V) at V ds = 3 V of this FET, the red locus of points represents the curve after annealing under condition of no threshold voltage (V T), and black locus of points represents the curve before annealing(V T = 15 V), respectively.
For all hybridizing taxa that differ in red pattern, there was a clear peak of absolute and relative divergence near the red locus (Additional file 4: Figure S3A).
In Figure 4, the single-link algorithm obtained the peak F1 score (0.915) with distance threshold 0.2 (the second point from the right on the red locus).
For example, selection against the introgression of the postman haplotype at the red locus from the postman race into the rayed race in a hybrid zone could result in genetic hitchhiking of neutral alleles at several sites near the red locus.
Similarly, selection against the introgression of the postman haplotype at the red locus from the postman race into the rayed race in a different hybrid zone could result in genetic hitchhiking of neutral alleles at several sites near the red locus that could be different from the sites that experienced genetic hitchhiking in the first hybrid zone.
This could result in a relative excess of allelic differences at several sites near the red locus between the similarly colored races at the two hybrid zones, as we observed in the allopatric postman comparisons.
We propose that selection at each hybrid zone may have lead to the fixation of different neutral alleles near the causative sites of the red locus, at each hybrid zone.
However, this cannot explain the allopatric rayed race comparisons where we found a reduction of absolute divergence near the red locus (Additional file 4: Figure S3D), and only a slight peak of relative divergence (Fig. 4b).
Among the 56 Tau and 19 Phi GSTs in sorghum, 44 Tau (78%) and 12 Phi (63%) GSTs are found in tandem clusters, respectively (red locus name in Supplementary Fig. S4; Fig. 2), indicating tandem duplications as the main mechanism for the Tau and Phi GST expansion.
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