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(C) Collagen production capacity of mFBs was measured by reading the absorbance of Sirius red labeled protein specimens.
Why are there so many red labeled cells in the hilus and outer GCL in Bax mutants treated with DMOG?
(K ) Section through red-boxed area of embryo MM9 reveals red labeled cardiomyocytes (asterisks) and green labeled endocardial cells arrows).
(B ) Quantification of type IIX pure (green only) and hybrid (green and red, labeled with asterisks) myofiber percentages.
However, we found very little co-localization of the green labeled mitochondria and red labeled RNA species.
(F ) Example image of VAMP2-mCherry + Munc13-KD synaptic terminals (red) labeled with Alexa488 anti-CaV2.1 (green).
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FITC-labeled and Texas-red labeled secondary antibodies (Jackson Labs, West Grove, PA, USA) were used for fluorescent microscopy.
The In situ Cell Death Detection Kit (TUNEL) with TMR-red labeled dUTP was purchased from Roche (Indianapolis, IN, USA) and used according to the manufacturers' instructions.
Visualization of vasculature was achieved by the i.v. injection of 250 µg of 70 kDa Texas-Red labeled dextran in PBS (Invitrogen).
Texas-Red labeled Sprague Dawley BBB mapping was financially supported by NIH K01 (Grant 1K01NS066131).
Unlabeled fibronectin was from Millipore and Texas-Red labeled fibronection was from Dr Sottile (University of Rochester).
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