Sentence examples for red incubation from inspiring English sources
Several Nile red incubation times are proposed in the literature.
Similarly, variation in the maximum emission intensity was found with time and algal strain (Nannochloropsis sp. or Tetraselmis sp ., leading to the recommendation to use the largest fluorescence intensity signal following Nile red incubation lasting from 30 to 40 min [ 23].
The maximized efficiency of intracellular lipid staining was achieved by adding glycerol to the Nannochloropsis sp. suspension to a final concentration of 0.1 g mL−1 (with a Nile red incubation of 5 min at room temperature in darkness and a Nile red concentration of 0.3 μg mL−1): fluorescence intensity with glycerol treatment was sixfold higher than without glycerol.
A 10-min incubation period is largely used with Nile red in the literature [ 25, 39, 47, 50, 94]; some authors established that this Nile red incubation time is optimal to avoid fluorescence fading in Chlorella vulgaris and Dunaliella salina [ 39, 84].
For the alizarin red staining, incubation of the samples was done in 50 mg/L Alizarin Red S (Gurr Searle Diagnostic, UK) in 2% KOH (pH 4,1 4.3; Sigma-Aldrich), during 2 8 hours at room temperature.
The dominating metabolites in red wine incubation analysed by targeted GCxGC-TOFMS were 3- 3′-hydroxyphenyl) propionic acid, 2-(3- 3′-hydroxyphenyl) acetic acid and benzoic acid, but 3- 3′-hydroxyphenylcid was also significantly above the control, suggesting compropionicydroxylacidn of 3-(3′-hydroxyphenyl) propionic acid.
After antibody incubation, red cell lysis and washing steps, cells were fixed.
After 2 h of incubation, red fluorescence was detected in the cells treated with the fluorescent particles (NC-RS100, LNC-PCL, and NC-S100) (Figure 7B,C,D).
The results of blood coagulation routine, hemolysis testing, red blood cell incubation experiment and blood absorption testing indicated that EDHSS had good hemocompatibility and no significant side-effects on the anticoagulation of whole blood.
After 6 h incubation, red fluorescence due to Alexa546-labelled ON705, delivered either with Nx+20 (A) or with Nx− 40 (B), was visible both in cytoplasm and in nuclei (arrows).
The potential to give rise to fully differentiated adipocytes from all preadipocyte cell lines used in this study was confirmed by lipid droplet staining with Oil red O after incubation with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine as described previously [19].
