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Camp Motion Pictures, which specializes in '80s DIY cinema, is selling a five-movie boxed set of schlock horror that includes a red cassette copy of Timothy O'Rawe's never-before-released "Basement" (1989), an anthology of horror shorts similar to "Tales From the Crypt".
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To determine whether repair of the NHEJ-red cassette requires XRCC4 we first knocked down XRCC4 by siRNA followed by genomic qPCR repair assay.
To induce cleavage of NHEJ-red cassette, Ad-SceI (30-100 MOI) was added to the culture medium and cells incubated with virus while slowly rocking for 6 h at 37°C, and then cells were collected at indicated time points for repair analysis.
The same PDGF-B cDNA was inserted also into the blunted PmeI/SfiI sites of the pCAG Ds-Red vector (kindly pCAG Ds-Red Dr. M. Goetz, Institute of Stem Cell Research, Germany), upstream the IRES-Ds-Red reporter cassette to form the PDGF-B/Ds Red vector.
This assembled attP1-SA-STOP-SV40-3xP3-dsRed-attP2 cassette was amplified with primers XZ195 and XZ196 and blunt cloned into pJET1.2 to generate pJET1.2-STOP-dsRed.
He po red over bootlegged cassettes, with no context as to where they came from, found at shops that sold them illegally.
In this construct, the DAT gene was fused to the 3′ end of the tR2AU cassette (turbo red fluorescent protein from Entacmaea quadricolor, the 2A sequence of porcine teschovirus, and human ubiquitin sequences).
One can mouse over or click the red dotted line box surrounding a cassette to see the cassette details discussed above and illustrated in Figure 1 ii).
#Assemble transfer cassette: a. red pole < clear plate < pad < 2 × 3 MM filter paper < membrane < gel < 2 × 3 MM filter paper < pad < black pole.
Transfer buffer: 38 mM glycine, 47 mM Tris, 11 mM SDS, 20% MeOH f. #Assemble transfer cassette: i. red pole < clear plate < pad < 2 × 3 MM filter paper < membrane < gel < 2 × 3 MM filter paper < pad < black pole.
The mT/mG reporter mouse possesses loxp sites at both sides of a tdTomato (mT) cassette and expresses red fluorescence in all tissues.
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