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Another recurrent sampling problem in empirical systems is that sampling is almost always unbalanced among host species (Biek et al. 2012; Higgins et al. 2012).
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No mutations were found in six recurrent samples.
Four recurrent samples were sequenced with none containing mutations.
Recurrent samples included fine needle aspirations, core and excisional biopsies.
Conversely, recurrent samples wild type for this mutation showed no staining.
A result for only one recurrent sample was obtained, which was negative for CTNNB1.
Results for both methods were only obtained for 12 primary and two recurrent samples.
Differences in non-silent mutation rate between untreated samples and recurrent samples treated prior with temozolomide chemotherapy were not found.
Importantly, in the present work, the expression of CD11b+ cells in the recurrent samples differed from CD68+ and CD163+.
The genomes of the primary (E1p) and recurrent sample (E1r) of relapse pair R2 were both balanced.
Unlike cases LGSC-9 and LGSC-12, sequencing of 4 primary and 4 recurrent samples revealed extensive mutational variability.
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