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Thus, for I227C channels the rectification index is 0.064 and 0.481 in the absence and presence of 100 µM Cd2+, respectively.

The rectification index was calculated as the ratio of the current amplitude measured at −140 mV to that measured at −5 mV.

Inward rectification (IR) index was calculated by dividing the absolute amplitude of average EPSC measured at −60 mV by that at +40 mV.

Rectification index (RI) was determined as the mean amplitude of the mEPSC at positive holding potential (+50 mV) divided by the mean amplitude of the mEPSC at negative holding potential (−70 mV).

Fast inward rectification (rectification index, RI) was measured by comparing the maximal membrane potential changes as responses to current pulses of −10 pA with those to −100 pA.

To quantify the inward rectification, we calculated the rectification index (RI), which is the ratio of the slope of the I V curve at positive divided by the slope at negative potentials.

The rectification index (Iins-60 mV/I60 mV) was 1.13 ± 0.06 (n = 8) (Fig. 1c), suggesting that the pore conducts macroscopic current approximately equally in both directions.

The sag index is an indicator of slow inward rectification.

In contrast, neurons expressing the Arp2/3 non-binding mutant, W413A-PICK1, showed no change in AMPAR EPSC amplitude, and no change in rectification index, indicating that interaction with Arp2/3 is essential for PICK1-mediated regulation of basal synaptic transmission.

Usually for rectification, full-wave rectification is preferred.

For rectification analysis, a ratio of the I/V plot slopes between −70 mV and 0 mV and between 0 mV and +40 mV data was calculated to generate a rectification index (RI=Gradient+40 mV/Gradient−70 mV).