This appears to be a cell cycle regulated process since when the nucleolar function is established during interphase, recruitment of processing proteins is not associated with PNB formation.
Given the importance of FADD to complex formation we reasoned that knockdown of FADD should block pro-CASP8 recruitment and processing.
It has been demonstrated that increasing the size of cortical representational maps likely provides recruitment of processing resources to increase tactile spatial acuity (Godde et al., 1996; Dinse et al., 1997).
For instance, in response to stress, the mitogen-activated protein kinase/p38 kinase signaling pathway mediates phosphorylation of serine-387 in AGO2, the core component of RISC, increasing its recruitment to processing bodies [ 9].
In the first model, conformational changes during a cycle of substrate turnover expose the Rpn10-binding site on Rpn12 to facilitate Rpn10 binding and hence substrate recruitment and/or processing.
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Thus, it seems that recruitment of the processing machinery at the time of nucleolar assembly is a regulated process most probably dependent on cell cycle progression.
