Sentence examples for recovery buffer from inspiring English sources

A total of 400 µl of recovery buffer was added to the disrupted gel pieces, and the samples were kept overnight at 4°C.

Cryogenically preserved human hepatocytes (~ 10 × 10 cells/sample) were obtained commercially, thawed, and equilibrated in the recovery buffer as recommended by the supplier.

The sheared gel pieces were covered with 300 μl DNA recovery buffer (8 mM Tris pH 8.0, 0.08 mM EDTA, 1.25 M ammonium acetate), vortexed, and eluted at 4°C overnight, followed by 15 minutes incubation at 65°C.

8 uL 0.1 M EDTA was added to each PCR product and the triplicate amplification reactions were pooled into a Clean-Up Plate (Clontech), washed 3 times with water, and eluted with 50 uL Recovery Buffer (Clontech).

The size fractions were sliced out of the gel and the DNA was mechanically sheared and and eluted over night in 300 μl recovery buffer (8 mM Tris pH 8.0, 0.08 mM EDTA, 1.25 M ammonium acetate. After a 15-min incubation at 65°C, the eluent was purified using a Montage DNA Gel Extraction Device (Millipore Corporation, Bedford, MA) and precipitated with isopropanol.

A number of recent studies report improved identification of proteins from FFPE tissue using these AR-based methods, which employ combinations of heat and recovery buffers containing Tris-HCl [15], detergents[16] [18] and reducing agents such as DTT [19], [20].

The degree to which reserve-driven rates of recovery will buffer the anticipated rise in rate of coral bleaching, disease, and severe hurricanes is currently unclear and will undoubtedly vary regionally [29].

Therefore, we need to determine an optimal recovery policy and buffer time allocation to the ship route in order to minimize the total costs associated with delays and recovery actions, such as increasing sailing speed.

The developed MIP shown as a material with specific binding to AD, comparing to its structural analogues, 1- 2-diethylaminoethoxy -2,6-diiodo-4-nitrobenzene and lidocaine, which shown 9.9% and 25.4% of recovery from the buffer solution, correspondingly.

(A ) Neuronal cultures were stimulated at 80 Hz for 10 s and then allowed to recovery in resting buffer for 30 min. CTX-HRP was added either during both stimulation and recovery, or during recovery only, as indicated.

Neurons of the indicated genotypes (A, WT and Dyn1 KO; B, Dyn3 KO and Dyn1/3 DKO respectively) were stimulated with 90 mM K+ for 90 s in the presence of HRP, followed by wash-out of HRP and recovery in resting buffer.