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The Strata-X cartridge was rinsed with sterile Milli-Q water, and [68Ga DOTATATE)] was subsequently recovered with ethanol (500 μL).
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The supernatant underwent a second precipitation, to recover chondroitin, with ethanol up to 4 volumes at 4 °C o/n and the recovered precipitate was dried at 40 °C under vacuum.
The residual IL was removed and pretreated biomass material was recovered with a series of water and ethanol washes.
After serial wash steps, immunoprecipitated DNA was recovered with overnight incubation at 65°C water bath followed by ethanol precipitation.
The lipoprotein samples were recovered with a syringe and delipidated in diethylether/ethanol (3:1, v/v) as previously described [29].
cDNAs are then blunt ended using T4 DNA Polymerase (Promega), extracted with phenol/chloroform/isoamylalcohol (25:24:1) and recovered by ethanol precipitation with ammonium acetate.
After the adsorption, Ile-Trp was recovered by elution with ethanol.
Reactions were recovered by precipitation with ethanol.
Total RNA was recovered by precipitation with ethanol and cleaned up by using the RNAsy kit (Qiagen).
Enzyme was removed by phenol choloroform extraction, and the DNA was recovered by precipitation with ethanol and dissolved in TE.
RNA was eluted overnight with 0.4 M NaCl at 4°C and recovered by ethanol precipitation with glycogen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com