Exact(4)
At the designated embryonic day point (from E10 – E15 for these experiments) mothers were sacrificed and embryos were recovered live.
Flies can be recovered live after recording by placing the chamber on a diffusive CO2 pad to anaesthetize flies.
To overcome this limitation, we employed electroporation as a means to deliver the CRISPR/Cas9 components, including Cas9 messenger RNA, single-guide RNA, and donor oligonucleotide, into mouse zygotes and recovered live mice with targeted nonhomologous end joining and homology-directed repair mutations with high efficiency.
To address whether the ZEN method can generate mice with precise genome modifications, we coelectroporated Cas9 mRNA, sgRNA targeting Tet2, and a 126-nt donor oligonucleotide with DNA sequence flanking the target site and a 2-nt mutation converting the naturally occurring EcoRV site (GATATC) in the target sequence to an EcoRI site (GAATTC) and recovered live mice.
Similar(56)
Consistent with previous studies on early death of Rb/p107 double mutant embryos [42], [49], we were unable recover live E14.5 E16.5 mgRb Rb−/−:p107−/− double mutant embryos after breeding mgRb Rb+/−:p107+/− mice (not shown).
He travelled extensively after recovering, living off a meagre RAF pension, and was persuaded to set down his experiences by the poet Robert Graves, who he met while touring Majorca with his wife, the poet Ruth Fainlight.
Out of pride I rejected the offer and spent the time I would have spent recovering, living in Uruguay: a happy land full of voluptuous woman and very appreciative men.
But, as Whetstine's research shows, in the age of organ transplantation, recovering living organs from clinically dead bodies raises conceptual problems [ 1].
Lady Gaga has torn soft tissues in her hip joint and the injury has forced her to cancel the rest of her Born This Way Ball tour to have surgery and recover, Live Nation Global Touring announced Wednesday.
The need for improved models to simulate chemical enhanced oil recovery (CEOR) methods involving either free gas or solution gas has increased in recent years as it has become more common to inject surfactants to recover live oils using surfactant-polymer or alkaline-surfactant-polymer flooding, or to inject gas with the surfactant solution (low-tension gas flooding or foam).
We present the first reported attempt to recover live vaccinia virus from the homes of recently vaccinated persons.
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