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The animals were recovered, individually housed, and offered water and food ad libitum.
For clonal assays, at day 13 for R-cells or day 16 for L-cells, cultures containing >10 30 colonies/plate, separated by a distance ≥2 round-shaped colony diameters, were washed in CMF-PBS and recovered individually.
For the genomic DNA extractions (gDNA), hemocytes were recovered individually as described above from three oysters used for gene quantification and from one oyster used for gene structure determination originating from the Mediterranean Sea (Thau lagoon).
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Rats then recovered in individually housed sterilized, standard rodent cages.
The corresponding precipitates were recovered, dissolved individually in fresh buffer and assayed for both total protein content and cellulase activity.
Once you obtain bacterial transformants after the second round, pick at least 50 colonies, recover plasmids individually and sequence.
Once fully recovered, animals were individually moved to clean cages and maintained in a pathogen-free facility.
We screened a retrovirus-encoded library of 8000 human open reading frames (ORFeome 1.1) (Rual et al., 2004) and individually recovered stably integrated ORFs from 732 resulting macroscopic colonies by PCR, followed by determination of the ORF identity by sequencing.
For TEM observations, larvae were dissected in PBS at different times after injection (30 min, 1, 2, 3, 4, 6, 8 and 15 hours) and the beads were individually recovered.
For confirming the accuracy of InDel loci in the transcriptional regions, the amplification products from 9 InDel primer pairs with different types were individually recovered from polyacrylamide gels and sequenced.
As shown in Fig. 5 c, both Rx1 and Rx2 obtain contaminated bits and cannot recover these bits individually.
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