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Afterwards, the products were recovered, gently washed with deionized water and dried at 60 °C for 4 h.
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Capital expenditure, which had been recovering gently since 2002, increased substantially between the third and fourth quarters of last year.
After 3 hours contact, attached but not internalized promastigotes were recovered by gently media aspiration.
Lavage fluid was recovered by gently flattening the inflated lobes and stored on ice until titered.
After 24 h, recruited cells were recovered by gently lavaging the pouch twice with 0.75 ml of PBS containing 1 mM EDTA.
I spend the rest of the afternoon in the kennels, checking that the dogs are recovering and gently scratching their noses through the bars.
The recovered supernatant was gently mixed with an equal volume of −20°C isopropanol.
The bottom phase containing lipids was gently recovered using a micropipette, dried, and resuspended in 350 μL of acetonitrile/water (3:2, v/v).
The PMNs were gently recovered and washed with PBS (without calcium or magnesium), suspended in PBS (2×10 cells/ml), counted and their viability was determined by Trypan Blue exclusion.
Testis contents from 2- to 3-mo old rats were gently recovered through an incision in the tunica albuginea and suspended in 25 ml of 0.01 M PBS (pH 7.2) containing 0.1% glucose, 3 mM lactate (PBSGL), and were incubated with collagenase II-S (12.5 mg in 25 ml of PBSGL) for 10 min at 33°C in a shacked water bath (150 rpm).
SP-4ME was cultured in similar conditions to those described for SP-4B, except that medium was partially exchanged daily from the day 3 onwards as follows: fifty percent of culture media was collected in sterile conditions and centrifuged at 200 × g for 5 min; the supernatant was discarded and the recovered cell aggregates gently resuspended in an equivalent volume of pre-warmed complete DMEM-HG.
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