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The transfection with siKGFR significantly inhibited cell migration induced by KGF (Fig. 4B) and subsequently reduced the recovered area from 96.4% of the control cells to 24.6%, 3.9 fold difference, P = 0.009) (Graph Fig. 4B).
The recovered area was calculated using ImageQuant TL (GE Healthcare).
The recovered area was calculated by ImageJ software for 3 experiments and results are shown by the mean ± s.e.m.m
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"Disturbances are very important; they are huge," said Mark Swanson, a Washington State University ecologist who recently published a paper noting that recovered areas thrived after the eruption of Mount St. Helens in 1980.
Recovered areas in the matrix appear both in the transverse and rolling directions around the particle.
"It is critical that they now secure roads to newly recovered areas to enable commercial traffic and humanitarian access".
A once severely degraded, but now recovering area is Dilamo micro-watershed of Kota Kombola kebele where privately owned land during Imperial government was converted to common land during the Derge government.
Interestingly, these population shifts are likely to be self-reinforcing, as inmigration supports local housing markets and businesses, thereby widening the gap between the recovering areas and the laggards.There were some surprises in the data, as well.
The operation aims to recover areas that have fallen to rebels.
Scratched areas were measured with ImageJ software (NIH, Bethesda, USA) and recovered surface area over 12 and 24 h was calculated compared to untreated cells.
Migration was quantified by a measure of the recovered wound area, performed using the freely available image-processing software ImageJ 1.38 (http://rsb.info.nih.gov/ij/).
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