Sentence examples for recovered and edited from inspiring English sources
Sequences were recovered and edited using MacVector (MacVector Inc .. ClustalW alignment was refined by hand, which made it possible to consider insertion polymorphism as well as mobile element insertions.
Hogan recovered and bodyslammed Earthquake.
Kershaw recovered and avoided surgery.
Differential DNA denaturation PCR was initially used to recover A3 edited viral genomes and plasmid DNA (Vartanian et al, 2008, 2010; Suspène et al, 2011a, 2011b).
Twenty-four F2's derived from positive F1's were screened singly by PCR or by visual inspection for GFP fluorescence to recover and propagate the edits.
Two recent papers are noteworthy because they used 3DPCR to recover lightly edited DNA sequences manifesting ∼2 13 CG->TA transitions per kilobase of nuDNA (Shinohara et al, 2012; Burns et al, 2013a).
Interview was condensed and edited.
SNPs missed by false alignments can be recovered by manually editing the problematic alignment.
Together, these results demonstrate that a co-conversion approach using a temperature-sensitive mutant allele, PCR-generated sgRNA templates, and NHEJ inactivation by RNAi provide a flexible, robust platform to recover genome editing events.
In another experiment, we co-injected three ssODNs carrying insertions of two different sizes (42, 42, and 66 nucleotides, experiment 8) and recovered all three edits.
