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Action potential stimulation for the autaptic recordings was achieved by 1.5 ms voltage pulses from −70 mV to 0 mV [39].
An accurate temporal alignment of video data with electrode recordings was achieved by synchronizing video frames of bat landing events with the acoustic artefact generated during landing manoeuvres of bats, which were clearly visible in the recordings of the technical bat detector.
Synchronization of confocal images and ionic current recordings was achieved using a Leica DAQ box and HEKA patch-master software (HEKA Elektronik), which delivered the triggers for confocal image acquisition.
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Older and younger (3 5 months) rats were equipped with electroencephalographic (EEG) and electromyographic (EMG) electrodes and polygraphic recordings were achieved under basal conditions with a digitized set-up.
After mice recovered from the surgeries and stable recordings were achieved (usually 1∼2 weeks post-surgery), we began the experiments.
Once stable whole cell recordings were achieved with good access resistance (usually <20 MΩ), the microscope objective was switched from 60× to 4× for laser scanning photostimulation.
Then the same adapting protocol was repeated a second time (Adaptation II, Fig. 1) and recordings were achieved a last time.
Sound recordings were achieved in two Tanzanian coastal forests located in the Rufiji valley (Rufiji District) and distant of 50 km.
Thus, anatomical reconstructions and electrophysiological recordings were achieved with different SAI afferents.
Perforated patch recordings were achieved by addition of 25 50 μg/ml amphotericin B (Sigma Aldrich) to the pipette solution.
Continuous pulse oximetry (SPO2) and ETCO2 recordings are achieved via couplings to the Marquette Solar 8000 monitor.
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